Technical and professional Histology services

Histology

Clients may submit tissue blocks for microtomy and/or staining only to Eurofins Pathology. Clients may submit unstained slides for staining only.

Clients may submit pathology material for Consultant Services. In some instances, routine histology technical work up may also be carried out in Eurofins Pathology prior to submission to a Consultant Pathologist.

Sample Type:

*Any unstained slide cases for staining only must be accompanied by

HLF64 Blocks and Slides Chain of Custody Log or, an approved local version
Clear identification of case number on slide

*Any block cases for microtomy and staining only must be accompanied by

HLF64 Blocks and Slides Chain of Custody Log or, an approved local version
Clear identification of case number on block

*Any block cases for microtomy, staining and reporting should be accompanied by

HLF64 Blocks and Slides Chain of Custody Log or, an approved local version, and a Test Request Form with gross description
Previous history where available

Any slide cases for reporting only should be accompanied by the following:

HLF16 Chain of Custody Log or an approved local version
A copy of gross report and test request form
Previous history where available
Clear identification of case number on slide

Turnaround Time:

As per service level agreement with client.

Sample Stability:

Slides, and specimen tissue blocks are both stable at room temperature. They should be kept out of direct sunlight.

Slides should be securely placed and sealed in a slide mailer or slide tray.

Specimen tissue blocks should be wrapped in a piece of tissue.

Instrument / Procedure:

Histology Processing. Diagnosis.

Units:

Reference Range:

Precautions:

Unstained Slides:

Sections must be mounted on a glass slide suitable for the tissue type and test required.
Sections must be mounted on the slide such that there is sufficient space for a control section at the foot of said slide if needed.
Slides must be clearly labelled in pencil.
Slides must not be heated / baked prior to sending
Where possible an extra unstained slide should be submitted on each case.

Stained Slides:

Slides should be clearly labelled, stained, and coverslipped.
Slides should be accompanied by:
- the case report when submitting slides for second opinion
- the gross report and test request form when primary reporting is required.

Paraffin Blocks (FFPE)

An adequate amount of tissue should be present within the block to account for alignment and cutting on Eurofins Pathology microtomes.

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Source:

Accreditation Status:

Accredited

Non-Accredited

SAMPLE REQUIREMENTS FOR COAGULATION TESTS

PROCEDURE

Sample Requirements and Collection

Patients should be relaxed pre-venepuncture. Excessive stress and exercise will increase FVIII, vWF antigen and fibrinolysis. Venous occlusion should be avoided.
Difficult venepuncture with trauma may lead to platelet activation with release of PF4 from alpha granules.
Venous blood should be collected into coagulation tubes containing Sodium Citrate 3.2%, 0.105M.
Specimens must be mixed immediately post venepuncture to avoid clot activation, by GENTLY inverting the tubes 5 to 10 times.
The ratio of whole blood to anticoagulant is crucial to clotting times. A target blood to anticoagulant ratio of 9:1 is optimal. Under- or over- filled specimens will not be processed this can adversely affect results.
Any warfarin treatment should be mentioned on the request form.
Sample rejection Criteria: Clotted sample, grossly hemolyzed sample, underfilled/overfilled specimen, unlabeled sample, mismatched patient ID, aged samples, wrong sample tube (citrate tube only).

Transportation and Storage

PT/INR specimens should be transported to the laboratory at room temperature.
Coagulation specimens should ideally be analysed within 4 hours of collection. Where this is not possible, centrifuge specimens at room temperature (RT) @ 1500RCF for at least 15 minutes, and then carefully remove the plasma from the cells, transfer to a fresh plastic plain tube and freeze at -20oC.
Non-frozen coagulation specimens should be transported at RT ASAP to avoid deterioration of labile factors V and VIII.
Collection of blood through intravenous lines that have been previously flushed with heparin should be avoided. In the event blood is drawn from an indwelling catheter, the line should be flushed with 5ml of saline, and the first 5ml of blood or 6 times the line volume be drawn off and discarded before coagulation tube is filled.
Effect of freezing on Coagulation Specimens.
A 14days in-house study on the effect of freezing, on coagulation specimens at -20oC, showed that there was negligible and clinically non-significant effect of freezing on coagulation specimen results. Therefore frozen citrated coagulation samples are stable for 14 days at -20oC, post centrifugation. This study is available in-house for reference.

ESR Ref Ranges

Units of Measurement

MALE

FEMALE

>50 Years

mm/hr

0 - ≤12

0 - ≤15

<50 Years

mm/hr

0 - ≤8

0 - ≤10

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Download Sample Report

Analyte

Units of Measurement

MALE

FEMALE

WBC

10^9/L

4.0–10.0

4.0 - 10.0

RBC

10^12/L

5.0 ± 0.5

4.3 ± 0.5

g/dL

15.0 ± 2.0

13.5 ± 1.5

HCT

L/L

0.45 ± 0.05

0.41 ± 0.05

MCV

92 ± 9

MCH

29.5 ± 2.5

MCHC

g/dL

33.0 ± 1.5

PLT

10^9/L

280 ± 130

MPV

N/A

RDW

11.6 - 14.0

#Neut

10^9/L

2.0 – 7.0

(40 - 80%)

#Lymph

10^9/L

1.0 – 3.0

(20 - 40%)

#Mono

10^9/L

0.2 – 1.0

(2 - 10%)

#Eos

10^9/L

0.02 – 0.5

(1 - 6%)

#Baso

10^9/L

0.02 – 0.1

(<1 - 2%)

Analyte

Units of Measurement

MALE & FEMALE

WBC

10^9/L

Birth: 18 ± 8
Day 3: 15 ± 8
Day 7: 14 ± 8
Day 14: 14 ± 8
1 Month: 12 ± 7
2 Months: 10 ± 5
3–6 Months: 12 ± 6
1 Year: 11 ± 5
2–6 Years: 10 ± 5
6–12 Years: 9 ± 4

RBC

10^12/L

Birth: 6.0 ± 1.0
Day 3: 5.3 ± 1.3
Day 7: 5.1 ± 1.2
Day 14: 4.9 ± 1.3
1 Month: 4.2 ± 1.2
2 Months: 3.7 ± 0.6
3–6 Months: 4.7 ± 0.6
1 Year: 4.5 ± 0.6
2–6 Years: 4.6 ± 0.6
6–12 Years: 4.6 ± 0.6

g/dL

Birth: 18.0 ± 4.0
Day 3: 18.0 ± 3.0
Day 7: 17.5 ± 4.0
Day 14: 16.5 ± 4.0
1 Month: 14.0 ± 2.5
2 Months: 11.2± 1.8
3–6 Months: 12.6 ± 1.5
1 Year: 12.6 ± 1.5
2–6 Years: 12.5 ± 1.5
6–12 Years: 13.5 ± 2.0

HCT

L/L

Birth: 0.60 ± 0.15
Day 3: 0.56 ± 0.11
Day 7: 0.54 ± 0.12
Day 14: 0.51 ± 0.12
1 Month: 0.43 ± 0.10
2 Months: 0.35 ± 0.07
3–6 Months: 0.35 ± 0.08
1 Year: 0.34 ± 0.04
2–6 Years: 0.37 ± 0.03
6–12 Years: 0.40 ± 0.05

MCV

Birth: 110 ± 10
Day 3: 105 ± 13
Day 7: 107 ± 19
Day 14: 105 ± 19
1 Month: 104 ± 12
2 Months: 95 ± 8
3–6 Months: 76 ± 8
1 Year: 78 ± 6
2–6 Years: 81 ± 6
6–12 Years: 86 ± 9

MCH

Birth: 34 ± 3
Day 3: 34 ± 3
Day 7: 34 ± 3
Day 14: 34 ± 3
1 Month: 33 ± 3
2 Months: 30 ± 3
3–6 Months: 27 ± 3
1 Year: 27 ± 2
2–6 Years: 27 ± 3
6–12 Years: 29 ± 4

MCHC

g/dL

Birth: 33.0 ± 3.0
Day 3: 33.0 ± 4.0
Day 7: 33.0 ± 5.0
Day 14: 33.0 ± 5.0
1 Month: 33.0 ± 4.0
2 Months: 32.0 ± 3.5
3–6 Months: 33.0 ± 3.0
1 Year: 34.0 ± 2.0
2–6 Years: 34.0 ± 3.0
6–12 Years: 34.0 ± 3.0

PLT

10^9/L

Birth: 100 – 450
Day 3: 210 – 500
Day 7: 160 – 500
Day 14: 170 – 500
1 Month: 200 – 500
2 Months: 210 – 650
3–6 Months: 200 – 550
1 Year: 200 – 550
2–6 Years: 200 – 490
6–12 Years: 170 – 450

Reticulocytes

10^9/L

Birth: 120 – 400
Day 3: 50 – 350
Day 7: 50 – 100
Day 14: 50 - 100
1 Month: 20 – 60
2 Months: 30 – 50
3–6 Months: 40 – 100
1 Year: 30 – 100
2–6 Years: 30 – 100
6–12 Years: 30 – 100

#Neut

10^9/L

Birth: 4 – 14
Day 3: 3 – 5
Day 7: 3 – 6
Day 14: 3 – 7
1 Month: 3 – 9
2 Months: 1.0 – 5
3–6 Months: 1 – 6
1 Year: 1 – 7
2–6 Years: 1.5 – 8
6–12 Years: 2 – 8

#Lymph

10^9/L

Birth: 3 – 8
Day 3: 2 – 8
Day 7: 3 – 9
Day 14: 3 – 9
1 Month: 3 – 16
2 Months: 4 – 10
3–6 Months: 4 – 12
1 Year: 3.5 – 11
2–6 Years: 6 - 9
6–12 Years: 1 - 5

#Mono

10^9/L

Birth: 0.5 – 2.0
Day 3: 0.5 – 1.0
Day 7: 0.1 – 1.7
Day 14: 0.1 – 1.7
1 Month: 0.3 – 1.0
2 Months: 0.4 – 1.2
3–6 Months: 0.2 – 1.2
1 Year: 0.2 – 1.0
2–6 Years: 0.2 – 1.0
6–12 Years: 0.2 – 1.0

#Eos

10^9/L

Birth: 0.1 – 1.0
Day 3: 0.1 – 2.0
Day 7: 0.1 – 0.8
Day 14: 0.1 – 0.9
1 Month: 0.2 – 1.0
2 Months: 0.1 – 1.0
3–6 Months: 0.1 – 1.0
1 Year: 0.1 – 1.0
2–6 Years: 0.1 – 1.0
6–12 Years: 0.1 – 1.0

Reference Ranges:

Age

Absolute Reference Range

Age

% Reference Range

0 - 1 day

324 - 617 x10⁹/L

0 - 1 day

1.72 - 8.62%

1 - 5 days

85 - 400 x10⁹/L

1 - 5 days

1.9 - 9.1%

5 days - 1 mth

34.2 - 724 x10⁹/L

5 days - 1 mth

0.1 - 6.9%

1 - 3 mths

21.3 - 205 x10⁹/L

1 - 3 mths

0.1 - 6.27%

3 - 12 mths

8.0 - 171 x10⁹/L

3 - 12 mths

0.1 - 4.7%

1 - 3 yrs

55.6 - 120 x10⁹/L

1 - 3 yrs

0.35 - 2.95%

3 - 7yrs

16.4 - 120.7 x10⁹/L

3 - 7yrs

0.25 - 2.57%

Adult

35.2 - 122.8 x10⁹/L

Adult

0.75 - 2.7%

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